2024年3月17日发(作者：手机资讯网)

B27神经细胞生长添加剂 / B27 NeuroMix

B27 NeuroMix

50X 浓度

编号

体积

F01-002 10 ml

应用

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血清替代品

海马神经元生长

多种CNS神经元

神经细胞基础培养基的添加剂

特点

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神经细胞的不同生长因子

含特定抗氧化剂

含神经元所需的特定脂肪酸

成分

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Biotin

L-Carnitine

Cholesterol

Corticosterone

Ethanolamine

D(+)-Galactose

Glutathione (reduced)

Lecithin

Linoleic Acid

Linolenic Acid

Phosphatidylcholin

Progesterone

Putrescine

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Retinol

Retinyl Acetate

Sodium Selenite

T3 (Triodo-I-Thyronine)

DL-α-Trocopherol (Vitamin E)

DL-α-Trocopherol Acetate

Proteins:

Albumin, bovine

Catalase

Insulin

Superoxide Dismutase

Transferrin

产品规格

pH

内毒素

无菌

7.0 – 7.5

已测定

已检测

保存期 1 年

贮存条件

≤ -15°C

Reagent Series

Protocol for B27 NeuroMix

B27 NeuroMix

F01-002 10 ml

Cat. No. Volume

Applications

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Differentiation of ES cells into neuron lineage (neuron and astrocytes)

Differentiation of neural stem cells into astrocytes and neurons

Optimal growth and long-term survival of rat hippocampal neurons (foetal and adult)

Survival of neurons from embryonic rat striatum, substantia nigra, septum and cortex, and

neonatal rat cerebellum (foetal and adult)

Shelf life and Storage

B27 NeuroMix is stable for 1 year when stored at ≤-15°C.

Instructions for use

B27 NeuroMix is a 50 fold concentrate. Dilute B27 NeuroMix (50x) into the base medium 1:50. The

final concentration of B27 NeuroMix corresponds to 1x. For preparation of 100 ml medium add 2

ml B27 NeuroMix (50x) into 98 ml of the appropriate base medium.

Cell culture vessels must be coated with poly-D-lysine (0.05 mg/ml). If using in combination with

N2 Supplement add fibronectin at a final concentration of 5-10 µg/ ml directly to the medium.

For cultivation of foetal neurons

Add B27 NeuroMix (50x) to Neuronal Base Medium (add 0.5 mM L-glutamine) to a final

concentration of 1x. For initial plating of embryonic primary hippocampal neurons 25 µM (3.7 µg/ml)

glutamate must be added for the first 4 days. After initial plating no glutamate is necessary.

Change media every 3-4 days.

For cultivation of adult and postnatal neurons

Add B27 NeuroMix (50x) and G5 Supplement (100x) to Neuronal Base Medium AD (add 0.5 mM

L-glutamine) to obtain a final concentration of 1x.

For serum free growth of neuroblastomas

Add B27 NeuroMix (50x) to Neuronal Base Medium (add 0.5 mM L-glutamine and 25 µM (3.7

µg/ml) glutamate) to a final concentration of 1x.

For in vitro laboratory use or further manufacturing only. Not for human use.