2024年4月13日发(作者:)
蛋白质电泳实验流程
英文回答:
Protein Electrophoresis Experimental Procedure.
Materials:
Protein sample.
Electrophoresis buffer.
Polyacrylamide gel.
Electrophoresis apparatus.
Power supply.
Staining solution.
Destaining solution.
Procedure:
1. Prepare the polyacrylamide gel.
Dissolve the acrylamide and bis-acrylamide in the
electrophoresis buffer.
Add the free radical initiator and allow the gel to
polymerize.
2. Load the protein sample onto the gel.
Dilute the protein sample in the electrophoresis
buffer.
Load the sample into the wells of the gel.
3. Run the electrophoresis.
Connect the electrophoresis apparatus to the power
supply.
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