2024年4月12日发(作者：)

文献1,3丙二醇工艺流程

English answer：

1,3-propanediol (1,3-PDO) is a versatile platform

chemical that has gained significant attention in recent

years due to its potential use in the production of bio-

based plastics, solvents, and pharmaceuticals. The

conventional production of 1,3-PDO involves the chemical

synthesis of propylene oxide (PO), which is then hydrated

to form 1,3-PDO. However, this process is energy-intensive

and relies on fossil fuel-based feedstocks.

In recent years, there has been increasing interest in

the development of sustainable and cost-effective bio-based

routes for the production of 1,3-PDO. One promising

approach involves the fermentation of glycerol, a byproduct

of the biodiesel industry, by genetically engineered

microorganisms. This process has been extensively studied,

and several microorganisms, including Escherichia coli,

Klebsiella pneumoniae, and Bacillus subtilis, have been

shown to be capable of producing 1,3-PDO from glycerol.

The overall process flow for the bio-based production

of 1,3-PDO from glycerol can be summarized as follows:

1. Pretreatment of glycerol: The initial step involves

the purification and pretreatment of glycerol to remove

impurities and prepare it for fermentation. This may

involve filtration, centrifugation, and/or chemical

treatment.

2. Fermentation: The pretreated glycerol is then

fermented by genetically engineered microorganisms that

have been engineered to produce 1,3-PDO. The fermentation

process is typically carried out in a fed-batch or

continuous bioreactor under controlled conditions of

temperature, pH, and oxygen supply.

3. Downstream processing: After fermentation, the broth

containing 1,3-PDO is subjected to downstream processing

steps to recover and purify the product. This may involve

centrifugation, filtration, and/or distillation.

The bio-based production of 1,3-PDO from glycerol

offers several advantages over the conventional chemical

synthesis route. Firstly, it utilizes a renewable and

sustainable feedstock, thus reducing reliance on fossil

fuels. Secondly, the fermentation process is energy-

efficient and generates less waste. Thirdly, the use of

genetically engineered microorganisms allows for the fine-

tuning of the production process and optimization of 1,3-

PDO yield.

中文回答：

1,3-丙二醇工艺流程。

1,3-丙二醇（1,3-PDO）是一种用途广泛的平台化学品，近年来

由于其在生物基塑料、溶剂和药物生产中的潜在应用而备受关注。

1,3-PDO的常规生产涉及环氧丙烷（PO）的化学合成，然后水合形

成1,3-PDO。然而，此工艺能耗高，并且依赖于基于化石燃料的原

料。

近年来，人们对开发用于生产1,3-PDO的可持续且经济高效的

生物基路线越来越感兴趣。一种很有前途的方法涉及通过基因工程

微生物发酵甘油，甘油是生物柴油工业的副产品。该工艺已被广泛

研究，并且已证明包括大肠杆菌、肺炎克雷伯菌和枯草芽孢杆菌在

内的几种微生物能够从甘油中产生 1,3-PDO。

从甘油生物基生产 1,3-PDO 的总体工艺流程可概括如下：

1. 甘油预处理，初始步骤涉及纯化和预处理甘油以去除杂质并

使其准备好发酵。这可能涉及过滤、离心和/或化学处理。

2. 发酵，然后，经过预处理的甘油由经过基因工程改造的微生

物发酵，这些微生物经过工程改造以产生1,3-PDO。发酵过程通常

在受控的温度、pH值和氧气供应条件下在分批补料或连续生物反应

器中进行。

3. 下游处理，发酵后，含有1,3-PDO的肉汤经过下游处理步骤

以回收和纯化产物。这可能涉及离心、过滤和/或蒸馏。

从甘油生物基生产1,3-PDO与传统的化学合成路线相比具有几

个优势。首先，它利用可再生和可持续的原料，从而减少了对化石

燃料的依赖。其次，发酵过程能耗低，产生的废物更少。第三，使

用基因工程微生物可以微调生产过程并优化1,3-PDO的产量。