2024年5月7日发(作者：ps怎么安装字体)

谷歌+百度翻译，不完全翻译，仅供参考，如有错误，概不负责。

Agilent Hi-Plex Ligand Exchange Columns

安捷伦Hi-Plex配体交换柱

Installation Column Connections

Hi-Plex columns are supplied with industry standard end

fittings. Only compatible nuts and ferrules should be used.

Nuts should be tightened finger tight and then 1/4 turn

using

a wrench. To avoid loosening column end fittings,

wrenches

must be used only on the outer column end fitting, marked

A in Figure 1.

安装色谱柱

Hi-Plex色谱柱提供行业标准端配件。 只能使用相容的螺

母和套圈。

一般用用手指拧紧后再用扳手拧紧1/4圈。

为避免松动柱端配件，扳手只能在外柱端配件上使用，

标有A在图1中。

Figure 1. Wrench surface.

图1.

扳手表面。

Alternatively, the appropriate PEEK “Fingertight” fitting can be

另外，适当的PEEK“Fightertight”配件可以

used, ensuring that there is no dead volume between the tube

使用，确保没有管和接头底部之间无死体积。

and the end fitting bottom.

Shipping Eluent

运输洗脱液

Hi-Plex columns are supplied containing UHP water. Columns

提供的Hi-Plex色谱柱包含UHP水。

are securely sealed with end caps which must ALWAYS be

柱与端盖安全密封

replaced when the column is disconnected from the system to

当柱与系统断开时，必须始终更换，以防止柱干燥。

prevent the column from drying out.

Column Conditioning

Hi-Plex columns must be run at elevated temperatures

(see Table 1). The mobile phase should be run at 0.1

mL/min and the column heating device switched on. When

the column reaches the desired operating temperature the

flow rate may be increased gradually to the required level.

Care should be taken to ensure that the maximum flow rate

of the column is not exceeded. Hi-Plex columns should not

be subjected to sudden changes in flow rate.

Under no circumstances should the column heating device

be left switched on with no flow through the column.

柱调节

Hi-Plex 色谱柱必须在比较高的温度下运行（参见

表格1）。

流动相应从0.1 mL / min 开始、色谱柱加热装置应当打

开。 当色谱柱达到所需工作温度时再使流量逐渐增加到

所需水平。

应注意确保柱的流速不超过最大流速。

Hi-Plex 色谱柱不应该受到流量突然变化的影响。

在任何情况下，都不能打开柱加热装置，也不能没有流

动相经过色谱柱。

谷歌+百度翻译，不完全翻译，仅供参考，如有错误，概不负责。

Mobile Phases

流动相

All mobile phases used must be of high purity and should be

所有使用的流动相必须具有高纯度，应该是

filtered (< 0.5 μ

m filter) and degassed before use. The mobile

过滤（<0.5μm过滤器）并在使用前脱气。

phase should be degassed in the vessel used as mobile phase

流动相脱气在应在流动相储层的容器中进行。

reservoir. Water is more effectively degassed at elevated

水在高温时能更有效地脱气。

temperature. Hi-Plex resins may be used with up to 5% v/v

Hi-Plex 树脂可以在流动相中使用高达

5％v / v

alcohol, or up to 30% v/v acetonitrile in the mobile phase. The

醇，或高达30％v / v 乙腈。

system should be thoroughly purged with the mobile phase

在尝试连接色谱柱之前， 应当使用流动相先替换整个

before attempting to connect the column.

色谱系统。

Sample Preparation

样品制备

Sample solutions should be filtered prior to use. Solvent

样品溶液应在使用前进行过滤。

precipitation or extraction may be used to remove protein,

溶剂沉淀或提取可用于去除蛋白质，脂质和其他生物污

lipid, and other biological contaminants. Ion exchange should

染物。

be used to remove ionic contaminants where appropriate.

离子交换应该用于去除离子污染物。

Guard columns should be replaced on a regular basis to ensure

应定期更换保护柱，确保分析柱不受污染。

contamination of the analytical column does not occur.

Maintenance Column Efficiency Testing

It is recommended that a simple chromatographic test is used

to monitor the performance of the column on a regular basis.

Ideally, the test should replicate that of the enclosed Column

Performance Report. Minor differences in peak shape or

retention time may be due to variations in system

configurations and hold up volumes. In the case of major

differences, the column should be re-tested with fresh mobile

phase after checking the system thoroughly for possible

sources of loss of efficiency such as excessive lengths of

connection tubing, or injector faults.

Appropriate guard columns are strongly recommended in all

维护柱效率测试

建议采用简单的色谱试验定期监测色谱柱的性能。

理想情况下，测试应该复制所附的内容色谱柱柱效报告。

峰值形状或停留时间的微小差异可能是由于系统配置的

变化和体积的增加引起的。

在较大差异的情况下，在彻底检查系统后，应重新检查

新的流动相，以寻找可能导致效率损失的来源，如连接

管长度过大或喷油器故障。

在所有情况下强烈建议使用适当的保护柱。

在样品制备过程中应特别小心，以尽量减少引入颗粒、

离子或疏水污染物的风险。

cases. Extreme care should be taken during sample

Hi-Plex 色谱柱具有很强的弹性，有时可以恢复以下性能

preparation to minimize the risk of introduction of particulate,

损失（见“柱清理”）。

ionic, or hydrophobic contaminants.

Hi-Plex 是低背压柱。系统压力不应用作柱压力的指示

Hi-Plex columns are quite resilient and can sometimes be

器。性能，而不是压力，是柱失效的主要指标。

recovered following a loss in performance (see “Column

Clean-up” ). Hi-Plex is a low backpressure column. System

pressure should not be used as an indicator of column

pressure. Performance, not pressure, is the primary indicator

of column failure.