2024年4月11日发(作者：)

髓过氧化物酶测定方法

英文回答：

The determination of myeloperoxidase (MPO) activity is

an important method used in various research fields,

including immunology, biochemistry, and clinical

diagnostics. MPO is an enzyme found in the azurophilic

granules of neutrophils, monocytes, and some macrophages.

It plays a crucial role in the innate immune response by

catalyzing the production of hypochlorous acid and other

reactive oxygen species, which are involved in the killing

of microorganisms.

There are several methods available for measuring MPO

activity. One commonly used method is the

spectrophotometric assay, which involves the oxidation of a

substrate by MPO and the subsequent measurement of the

absorbance at a specific wavelength. The most commonly used

substrate for MPO activity measurement is o-dianisidine,

which is oxidized by MPO to form a colored product that can

be quantified spectrophotometrically. This method provides

a simple and sensitive measurement of MPO activity and is

widely used in research and clinical laboratories.

Another method for measuring MPO activity is the

fluorometric assay, which utilizes a fluorescent substrate

that is oxidized by MPO to produce a fluorescent product.

This method offers increased sensitivity compared to the

spectrophotometric assay and is particularly useful when

measuring low levels of MPO activity. The fluorometric

assay can be performed using a microplate reader or a flow

cytometer, depending on the specific experimental setup.

In addition to these enzymatic assays, there are also

immunoassays available for measuring MPO activity. These

assays utilize specific antibodies that recognize MPO and

can be used to quantify the amount of MPO present in a

sample. Immunoassays are often used in clinical settings

for the diagnosis and monitoring of inflammatory diseases,

such as rheumatoid arthritis and vasculitis.

中文回答：

髓过氧化物酶（MPO）活性的测定是在免疫学、生物化学和临床

诊断等各个研究领域中常用的方法。MPO是一种存在于中性粒细胞、

单核细胞和某些巨噬细胞的髓绿色颗粒中的酶。它通过催化次氯酸

和其他活性氧物质的产生，在先天免疫应答中发挥重要作用，参与

杀灭微生物的过程。

目前有几种可用于测定MPO活性的方法。其中一种常用的方法

是分光光度法，该方法涉及MPO对底物的氧化反应，并测量特定波

长处的吸光度。最常用的MPO活性测定底物是邻二甲氧基苯胺，它

被MPO氧化形成一种有色产物，可以通过分光光度法定量测定。这

种方法简单灵敏，广泛应用于研究和临床实验室中。

另一种测定MPO活性的方法是荧光法，它利用一种荧光底物，

该底物被MPO氧化生成荧光产物。与分光光度法相比，荧光法具有

更高的灵敏度，特别适用于测量低水平的MPO活性。荧光法可以使

用微孔板读数器或流式细胞仪进行，具体取决于实验设置。

除了这些酶学测定方法，还有一些免疫测定方法可用于测定

MPO活性。这些测定方法利用特异性抗体识别MPO，并用于定量测定

样品中的MPO含量。免疫测定方法常用于临床诊断和监测炎症性疾

病，如类风湿性关节炎和血管炎。

总之，髓过氧化物酶测定方法在各个研究领域和临床实验室中

都有广泛应用。通过选择适当的测定方法，我们可以准确地测量

MPO活性，并进一步了解其在疾病发生和发展中的作用。