2024年4月12日发(作者：)

组织工程骨软骨修复兔膝关节全层软

骨缺损的实验研究

摘要：

目的：本研究旨在探讨组织工程骨软骨（TEBC）修复兔膝关节

全层软骨缺损的有效性和安全性。

方法：选取16只新西兰白兔，分为两组，每组8只。分别建

立全层软骨缺损的兔膝关节模型，并分别进行TEBC和对照组

治疗。其中，TEBC治疗组使用人工软骨基质复合干细胞进行

修复，对照组则使用生理盐水进行治疗。术后定期进行临床表

现观察、关节损伤评分和影像学评估。

结果：治疗6个月后，TEBC治疗组显著优于对照组，从临床

表现、关节损伤评分和影像学评估等多个方面均表现出更佳的

修复效果。在TEBC治疗组中，干细胞成功分化为软骨细胞，

并有较好的成熟程度。治疗过程中未发现严重的不良反应。

结论：组织工程骨软骨治疗可有效修复兔膝关节全层软骨缺损，

并具有较高的安全性和可行性。TEBC治疗临床前景广阔，值

得深入研究和推广应用。

关键词：组织工程骨软骨；干细胞；兔膝关节；全层软骨缺损；

修复效果。

Abstract:

Objective: The purpose of this study was to explore

the effectiveness and safety of tissue engineered

bone-cartilage (TEBC) in repairing full-layer

cartilage defects in rabbit knee joints.

Methods: Sixteen New Zealand white rabbits were

divided into two groups of 8 each. Full-layer

cartilage defects were created in each knee joint of

the rabbits, and treated with TEBC or saline,

respectively. In the TEBC group, artificial

chondroitin sulfate was used for scaffold, combined

with stem cells for repair. In the control group,

normal saline was used for treatment. Clinical

manifestations, joint injury scores, and imaging

evaluations were regularly observed after surgery.

Results: After 6 months of treatment, the TEBC group

showed significantly better repair results than the

control group, from clinical manifestations, joint

damage scores, imaging evaluations and other aspects.

In the TEBC group, stem cells were successfully

differentiated into chondrocytes and had a good

maturity. No serious adverse reactions were found

during treatment.